Analysis Note

ControlMonkey brain tissue, rat brain lysate

Application

This Anti-Dopamine D2 Receptor Antibody is validated for use in ELISA, IH, IP, WB for the detection of Dopamine D2 Receptor.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Western blot:
1-10 µg/mL (Chemiluminescence technique) The predicted size of D2 is approximately 50 kDa. However, additional bands at approximately 68 and 100 kDa, and others are typically seen in various tissues (Boundy et al. 1993; Sakata et al. 1992; Farooqui et al. 199; Farooqui & Prasad 1992, BBRC; Brock et al. 1992; Farooqui & Prasad 1992, Life Sci). Approximately 100-400 µg of membrane protein/lane should be loaded to visualize various bands.

Immunohistochemistry:
2-10 µg/mL on paraformaldehyde fixed sections on a previous lot.
Immunohistochemistry:
1-20 µg/mL on paraformaldehyde fixed sections.

Immunoprecipitation:
1-10 µg/mL using 100-400 µg of protein on a previous lot. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms (2).

ELISA:
0.5-1.0 µg/mL (1 µg/mL immunogen peptide (Catalog number AG221)/well) of a previous lot was used on ELISA.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. 2 isoforms are reported for the D2 Dopamine Receptor, Isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.

Immunogen

A 28 amino acid peptide sequence from the human D2 receptor within the cytoplasmic
loop #3.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal liquid in PBS with 0.1% BSA.

ImmunoAffinity Purified

Quality

Routinely evaluated by Western Blot on human brain lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected Dopamine Receptor D2 on 10 µg of human brain lysates.

Specificity

Specific for Dopamine Receptor D2 short and long form. Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3 (1). D2L receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (2). The immunogen peptide shows no significant homology with other dopamine receptors (D1 and D3-D5).

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

50 kDa